Fig. 4.

Modeling bacterial genotypic and phenotypic conservation across an evolutionary gradient. The presence of orthologous proteins, TonB outer membrane receptors, cytochromes, methyl-accepting chemotaxis proteins (MCPs), transcriptional regulators, metabolic pathways, protein expression patterns, and reduction of metal or metalloids (anaerobic growth) was determined for the 10 Shewanella strains (see Materials and Methods). Each of the traits was compared among the Shewanella strains in a pairwise manner (45 comparisons in total). The fraction of shared traits was determined for each pair of strains and plotted against the average nucleotide identity (ANI) of the respective strain pair. Inset graph depicts the relationships between conservation of the traits and evolutionary distance using linear regression trendlines adjusted to intersect with the x and y axis at 100%. The r² values of the regressions are also shown.