Table 1.

Generation time (h) and relative growth yield (%)^* of M. barkeri Fusaro strains^† in various media

Strain	Substrates
	CH3OH	CH3OH/H2/CO2	H2/CO2	CH3COOH
Δhpt‡	7.3 ± 0.3 (100%)	5.7 ± 0.1 (100%)	13.7 ± 2.5 (100%)	37 ± 3.4 (100%)
Δfpo	7.3 ± 0.2 (101%)	6.1 ± 0.5 (100%)	11.9 ± 0.9 (100%)	36 ± 2.6 (100%)
ΔfpoF	7.3 ± 0.2 (96%)	5.8 ± 0.5 (96%)	12.4 ± 1.2 (90%)	39 ± 1.9 (84%)
Δfre	7.7 ± 0.3 (116%)	5.5 ± 0.4 (125%)	8.8 ± 0.8 (95%)	41 ± 3.1 (84%)
Δfrh	13.7 ± 0.6 (52%)	6.5 ± 0.3 (80%)	NG (NA)	55 ± 7.0 (84%)
Δfpo Δfrh	NG (NA)	5.0 ± 0.3 (80%)	NG (NA)	NG (NA)
ΔfpoF Δfrh	NG (NA)	6.4 ± 0.4 (73%)	NG (NA)	76 ± 0.8 (66%)

*Growth rate and yield were measured as described in Materials and Methods; growth yield is relative to the parental strain on the same substrate. Values represent the average and standard deviation of at least triplicate measurements.

^†Strains used were WWM85 (Δhpt), WWM71 (Δfpo), WWM123 (ΔfpoF), WWM122 (Δfrh), WWM108 (Δfpo Δfrh), and WWM145 (ΔfpoF Δfrh).

^‡M. barkeri Fusaro parent strain in which all deletions were constructed.

NG, no growth for at least 6 months of incubation; NA, not applicable.