Table 2.

Methane (μmoL) and carbon dioxide (μmoL) production^* from resting cell suspensions of M. barkeri Fusaro strains^†

Strain	Substrates‡
	N2		CH3OH		CH3OH/H2		H2/CO2
	CH4	CO2	CH4	CO2	CH4	CO2	CH4	CO2
Δhpt§	<1	2 ± 0.1	343 ± 4	108 ± 2	465 ± 17	<1	298 ± 21	NA
Δfpo	<1	1 ± 0.1	365 ± 3	115 ± 1	494 ± 11	<1	356 ± 6	NA
ΔfpoF	<1	1 ± 0.1	332 ± 7	106 ± 2	439 ± 27	<1	359 ± 9	NA
Δfre	<1	1 ± 0.1	399 ± 14	133 ± 4	508 ± 66	<1	381 ± 21	NA
Δfrh	<1	2 ± 0.9	149 ± 3	42 ± 1	481 ± 14	<1	7 ± 2	NA
Δfpo Δfrh	<1	2 ± 0.7	47 ± 2	9 ± 1	480 ± 59	<1	10 ± 1	NA
ΔfpoF Δfrh	<1	4 ± 0.1	54 ± 3	16 ± 1	465 ± 9	<1	11 ± 5	NA

*Values are the average and standard deviation of at least 3 trials.

^†Strains used were WWM85 (Δhpt), WWM71 (Δfpo), WWM123 (ΔfpoF), WWM122 (Δfrh), WWM108 (Δfpo Δfrh), and WWM145 (ΔfpoF Δfrh).

^‡ Assays were conducted as described in Supplementary Information.

^§M. barkeri Fusaro parent strain in which all deletions were constructed.

NA, not applicable (CO₂ produced could not be measured because it was added to headspace).