Cellular localization of autophagosomes during infection-related development of a ΔMoatg4 mutant of M. oryzae. (A) Conidia were harvested from a ΔMoatg4 transformant expressing a GFP:MoATG8 gene fusion, inoculated onto glass coverslips and observed by epifluorescence microscopy at the times indicated (Scale bars, 10 μm.). (B) Bar chart showing mean autophagosome numbers present in conidium, germ tube, and appressorium 0 h, 2 h, 4 h, 6 h, 10 h and 12 h after inoculation with ΔMoatg4 mutant expressing a GFP:MoATG8 (error bars indicate ± 2 SE).