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. 2009 Sep 1;106(37):15979–15984. doi: 10.1073/pnas.0908111106

Table 1.

Relative cyclic electron flow (CEF) and cellular ATP content in wild-type, stt7–9, dum22, and dum22 stt7–9 cells

Strain CEF ATP TAP Dark ATP TAP hν ATP Min hν
Wild type 0.35 ± 0.05 66.2 ± 6.8 100 93.0 ± 9.1
stt7–9 0.37 ± 0.05 55.9 ± 6.1 102.3 ± 12.3 83.8 ± 20.4
dum22 0.63 ± 0.09 3.0 ± 1.3 69.0 ± 10.0 69.0 ± 10.0
dum22 stt7–9 0.35 ± 0.02 1.8 ± 2.1 65.7 ± 6.5 71.2 ± 2.7

CEF was evaluated as = k * [P700+]/([P700+]+[P700] after illumination in the presence of DCMU (20 μM), where k represents the P700+ re-reduction rate after steady-state illumination of saturating intensity. CEF rates at low light (50 μmol m−2 s−1) are normalized to the maximum value (typically 12.5 ± 0.1 s−1 in wild-type cells). Standard deviation is relative to at least 5 replicates. ATP is expressed in percentage of the wild-type control (107 ± 12 nmol/mg Chlorophyll-1). Cells were fixed after 3 h in the dark or after continuous illumination with low light (50 μmol m−2s−1).