Table 1.
Strain | CEF | ATP TAP Dark | ATP TAP hν | ATP Min hν |
---|---|---|---|---|
Wild type | 0.35 ± 0.05 | 66.2 ± 6.8 | 100 | 93.0 ± 9.1 |
stt7–9 | 0.37 ± 0.05 | 55.9 ± 6.1 | 102.3 ± 12.3 | 83.8 ± 20.4 |
dum22 | 0.63 ± 0.09 | 3.0 ± 1.3 | 69.0 ± 10.0 | 69.0 ± 10.0 |
dum22 stt7–9 | 0.35 ± 0.02 | 1.8 ± 2.1 | 65.7 ± 6.5 | 71.2 ± 2.7 |
CEF was evaluated as = k * [P700+]/([P700+]+[P700] after illumination in the presence of DCMU (20 μM), where k represents the P700+ re-reduction rate after steady-state illumination of saturating intensity. CEF rates at low light (50 μmol m−2 s−1) are normalized to the maximum value (typically 12.5 ± 0.1 s−1 in wild-type cells). Standard deviation is relative to at least 5 replicates. ATP is expressed in percentage of the wild-type control (107 ± 12 nmol/mg Chlorophyll-1). Cells were fixed after 3 h in the dark or after continuous illumination with low light (50 μmol m−2s−1).