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. 2009 Aug 25;106(37):16003. doi: 10.1073/pnas.0908998106

Fig. 4.

Fig. 4.

Inhibition of aromatase activity impairs uterine decidualization. (A) The hormonal regimen used in the artificial decidualization protocol is shown. Mice were treated with or without letrozole (20 mg/kg body weight). Uteri were collected 72 h after the application of stimulus. (B) The extent of decidual response in ovariectomized mice treated with P (Left) and P plus letrozole (P+AI, Right) is shown. s and us denote stimulated and unstimulated uterine horns, respectively. (C) The quantitative analysis of the average weight gain of stimulated relative to unstimulated horns in mice (n = 5) subjected to artificial decidualization with or without letrozole treatment. The data are presented as mean ± SEM. (D) Uterine RNA was isolated 72 h after the initiation of decidualization and subjected to quantitative PCR analysis using gene-specific primers for ERα, alkaline phosphatase (Alkp), BMP2, connexin 43 (Cx43), and PRP. P and P+AI represent uterine RNA from ovariectomized mice treated with P and P plus letrozole, respectively.