Figure 6. Lineage marker expression in clonal XEN-P cell lines.

(A) Line RX1 was subcloned by stable transfection, and three different primary colonies were arbitrarily picked, expanded, and analyzed by indirect immunofluorescence (left; compare to Fig. 3) or RT-PCR (right; compare to Fig. 2C). (B) Line RX1 was subcloned by single-cell FACS deposition. 20 clones were analyzed for Oct4 expression (all positive), and 5 representative photos are shown.