Table 4. List of antibodies used in this study.

Antigen	Primary antibody and dilution*	Secondary antibody and dilution*
Oct4	SC-5279, 1∶100	SC-2068, 1∶1000; JI-115-036-003, 1∶10,000 (WB); SC-3699,1∶200; JI-115-486-003, 1∶200 (IF)
Oct4	SC-9081, 1∶200	SC-3842, 1∶300
Gata6	SC- 9055, 1∶200	SC-3842, 1∶300
Gata4	SC-9053, 1∶200	JI-111-036-003, 1∶10,000 (WB); JI-111-496-003, 1∶200 (IF)
Nanog	Ab-21603, 1∶200	SC-3842, 1∶300
Sox7	SC-20093, 1∶200	JI-111-036-003, 1∶10,000
Beta-Actin	A-2228, 1∶10,000	JI-115-036-003, 1∶10,000
SSEA-1	DSHB MC-480, 1∶500	SC-3699, 1∶200
SSEA-3	DSHB MC-631, 1∶100	I-81-6514, 1∶100
Laminin B2	DSHB D18, 1∶400	SC-3699, 1∶200
Collagen 4	DSHB M3F7, 1∶200	SC-3699, 1∶200

^*SC, Santa Cruz Biotechnology, Inc.; Ab, Abcam plc; JI, Jackson Immunoresearch; DHSB, Developmental Studies Hybridoma Bank, University of Iowa.