Table 1. eSRK chimeras assayed in planta.
| Functional Chimeras | Observed Specificity a | Swapped region (SRKa #s) b | No. of polymorphic sites within the swapped region c | No. of polymorphic sites outside the swapped region c |
|---|---|---|---|---|
| eSRKa(b)b | b | 198-403 (197-403) | 91 | 67 |
| eSRK16(b)16 d | b | 198-357 (197-357) | 70 | 91 |
| eSRKa(7)a | 7 | 196-351 (200-356) | 42 | 58 |
| eSRK16(25)16 | 25 | 197-355 (198-357) | 46 | 28 |
| Nonfunctional Chimeras | Expected Specificity | Swapped region (SRKa #s) b | No. of transgenic plants analyzed | |
| eSRKa(b)a | b | 198-357 (197-357) | 7 | |
| eSRK25(16)25 | 16 | 197-355 (198-357) | 16 | |
| eSRK25(16)16 | 16 | 197-401 (198-403) | 17 | |
| eSRKa(16)a | 16 | 197-354 (198-356) | 8 | |
| eSRKb(16)b | 16 | 196-355 (197-357) | 12 | |
| eSRK25(a)25 | a | 198-357 (198-357) | 13 | |
| eSRK16(a)16 | a | 198-356 (198-356) | 8 | |
SI specificity was determined by pollinating the stigmas of transgenic plants expressing each chimera with pollen expressing the SCR variant corresponding to the swapped hvI-hvIII region. The source of pollen was A. thaliana plants transformed with one of the SCR constructs diagrammed in Figure S1. Functional chimeras expressed the expected specificities. For nonfunctional chimeras, the indicated specificity was expected but not conferred in transgenic stigmas.
The numbers show the limits of the swapped region in each eSRK chimera (see alignments in Supplementary Figure 2), with the corresponding numbers in SRKa shown in parentheses for reference.
The eSRK16(b)16 chimera contains the smallest swapped region of all chimeras tested.
The numbers indicate the number of amino-acid differences between the pair of SRK variants used to generate each chimera.