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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Traffic. 2009 Jun 9;10(9):1318–1336. doi: 10.1111/j.1600-0854.2009.00955.x

Figure 11. Tyrp1 delivery to melanosomes is blocked by dominant negative inhibition of ESCRT function.

Figure 11

IFM analysis of 1011-mel cells transfected 24-36 hrs earlier with V5 epitope–tagged dnVps4 (a-f) or HA epitope-tagged Tsg101 (g-i) and costained with anti V5 and TA99 anti-Tyrp1 (b,d,h). Panels c,f and i, colorized and merged panels. Large arrow in d-f indicates a cell overexpressing dnVps4 as determined by triple labeling with goat-anti V5 antibody and detected by AMCA-conjugated anti-goat IgG (data not shown). Large arrow and star (*) in g-i indicate cells expressing modest and high levels of Tsg101, respectively. Insets are 2.5× magnification of boxed regions. Small arrows mark areas of colocalization. Bar, 10 μm.