Figure 2.

Carbachol elicits I_sADP by activating Gα_q-11. A₁–A₃, The ability of carbachol (30 μm) to induce the appearance of I_sADP in a pyramidal cell of prefrontal cortex in organotypic culture is blocked by administration of pirenzepine (1 μm), an antagonist of Gα_q-11-coupled M₁ muscarinic receptors. B₁, A burst of three depolarizing steps delivered in the presence of carbachol trigger I_sADP in a different pyramidal cell recorded in a cultured organotypic cortical brain slice (gray trace, control; black trace, 30 μm carbachol). B₂, The same stimuli fail to elicit an I_sADP in a pyramidal cell transfected with the Gα_q-11 dominant-negative PLCβ-ct.