Figure 7.
Expression of TRPC5 and TRPC6 facilitates IsADP. A1–A3, IsADP recorded in a control (untransfected) pyramidal neuron, a pyramidal neuron transfected with TRPC6, and a third pyramidal neuron transfected with TRPC5. B1, Flattened confocal image stack depicting a pyramidal neuron transfected with TRPC6. Scale bar, 100 μm. B2, B3, Neuronal firing elicited by depolarizing constant current pulses (0, 100, 350 pA) in a control (untransfected) pyramidal neuron and an adjacent cell transfected with TRPC6 (paired recording). During administration of carbachol the TRP6-transfected pyramidal neuron developed an IsADP in excess of 1 nA. C, Graph summarizing the effect of expressing TRPC5 or TRPC6 on the amplitude of IsADP in pyramidal cells of the prefrontal cortex. *p < 0.05.