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. Author manuscript; available in PMC: 2010 Feb 10.
Published in final edited form as: Biochemistry. 2009 Feb 10;48(5):839–849. doi: 10.1021/bi801885w

Figure 4.

Figure 4

PAGE assay illustrating the recognition and photocleavage of mismatches and abasic sites by Rh(bpy)2(chrysi)3+ and Rh(bpy)2(phzi)3+. 1 μM duplex was incubated without metal complex (lanes marked “-”) , with 1 μM Rh(bpy)2(chrysi)3+ (lanes marked “C”), or with 100 nM Rh(bpy)2(phzi)3+ (lanes marked “P”) in 50 mM NaCl, 10 mM NaPi, pH 7.1. Duplex identity is indicated at the top of the gel. Samples were irradiated for 10 min on an Oriel Instruments solar simulator (320-440 nm emission) and incubated for 30 min at 60 °C prior to electrophoresis.