Skip to main content
. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Cell. 2009 May 1;137(3):472–484. doi: 10.1016/j.cell.2009.02.039

Figure 2. Identification of an endogenous CENP-A prenucleosomal complex.

Figure 2

(A) Chromatin-free extract derived from HeLa cells was subjected to sucrose gradient sedimentation. The bottom of the gradient appears on the right. The migration of sedimentation coefficient standards bovine serum albumin, aldolase, catalase and thyroglobulin are indicated by arrows at the top. (B) A direct interaction was observed between recombinant GST-HJURP and recombinant untagged CENP-A:H4 but not recombinant His-H2A:H2B dimer. Following purification on glutathione agarose, proteins associated with GST-HJURP were separated by SDS-PAGE and stained with Coomassie blue.