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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Vascul Pharmacol. 2009 Jun 26;51(2-3):198–204. doi: 10.1016/j.vph.2009.06.005

Fig. 2. Expression of molecular markers in the left ventricle of hearts subjected to pressure overload hypertrophy.

Fig. 2

A, Northern blot hybridization of total RNA from the left ventricle of sham and TAC C57BL/6J mice shows an increase in expression of atrial natriuretic peptide (ANP) and skeletal α actin (Sk. α-actin). The bar graph shows the densitometric analysis of the relative ANP expression versus β-actin. Although the Northen blot shows samples from each group in duplicate, the relative values were obtained from experiments in triplicate, values are means ± s.e.m * p < 0.05 is statistical significant. B, as above, Northern blotting was done using the same DNA probes in RNA samples from left ventricle of ob/ob mice of sham and TAC groups. A blunted response of ANP expression during TAC is observed in ob/ob mice hearts. Chronic intraperitoneal administration of leptin triggered the expression of ANP in ob/ob mice subjected to TAC. Statistical analysis of the relative densitometric values for ANP expression versus β-actin was performed as before. The values were obtained from triplicate experiments. One way Anova and Bonferroni’s test were used. Values are means ± s.e.m * p < 0.05 is statistically significant. S, sham; TAC, trans-aortic constriction, TAC + L, trans-aortic constriction and intraperitoneal injection with leptin 0.3 mg/kg/ per day.