| L |
length of cilium. 40μm≤L≤50μm |
|
rc
|
ciliary radius. 0.15μm |
|
Ri
|
ciliary intracellular resistivity. 9.2×105 μm/S |
|
ra
|
ciliary intracellular resistance.
|
|
gCNG
|
single CNG channel conductance. 5.0×10−4 nS/ch |
|
gCl
|
single Cl(Ca) channel conductance. 8.0×10−4 nS/ch |
|
K1/2
|
half maximum concentration of Ca2+ to activate Cl(Ca) channels. 4.8μM |
|
Pmax
|
maximum open probability of CNG channels. 0.7 no units |
|
DCa
|
Ca2+diffusion coefficient. 300 (μm)2/s [1] |
| DB |
BAPTA diffusion coefficient. 95 (μm)2/s [8] |
| D |
approximated diffusion coefficient. 100 (μm)2/s |
|
k+
|
forward reaction rate constant. 600/μMs [8] |
|
k−
|
backward reaction rate constant. 100s−1 [8] |
| BT |
total concentration of BAPTA (complex plus free). 2000μM |
| q |
converting factor of transmembrane current.
[9] |
| α |
conversion factor for binding. 2.7×10−2 μMμm/molecule |
| BS |
number of binding sites. 1 molecule/ch |
|
fCNG
|
fraction of the CNG current carried by Ca2+. 0.4 no units [10] |
| fX |
computational parameter. 0.97 no units |
| gX |
single Na+/Ca2+ exchanger conductance. gX = fX fCNGgCNG Pmax = 1.3×10−4 nS/ch |
| K |
reaction dissociation constant.
|
|
TCNG
|
total number of CNG channels. Units ch |
|
TCl
|
total number of Cl(Ca) channels. Units ch |
| TX |
total number of Na+/Ca2+ exchangers. Units ch |
|
cBulk
|
free calcium concentration at the exposed end of the cilium. cBulk = 300μM for the diffusion experiment and for the interaction experiment cBulk = 0μM |
|
vBulk
|
voltage clamp. vBulk = −50mV for the diffusion experiment and for the interaction experiment vBulk = −40, −60 and −80mV |