Abstract
Superoxide dismutase (SOD) activity was demonstrated in cell-free extracts of Bacteroides fragilis, Bacteroides vulgatus, Bacteroides distasonis, Bacteroides ovatus, and Bacteroides thetaiotaomicron. The strains were grown under anaerobic conditions in Trypticase soy broth, and the specific activity of SOD in the extracts was, in most strains, higher than in cell-free extracts of Escherichia coli B grown under anaerobic conditions. Isoelectric focusing of the extracts in polyacrylamide gel demonstrated distinct forms of SOD in the different species.
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- Beerens H., Wattre P., Shinjo T., Romond C. Premiers résultats d'un essai de classification sérologique de 131 souches de Bacteroïdes du groupe fragilis (eggerthella. Ann Inst Pasteur (Paris) 1971 Aug;121(2):187–198. [PubMed] [Google Scholar]
- Carlsson J. Simplified gas chromatographic procedure for identification of bacterial metabolic products. Appl Microbiol. 1973 Feb;25(2):287–289. doi: 10.1128/am.25.2.287-289.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gregory E. M., Kowalski J. B., Holdeman L. V. Production and some properties of catalase and superoxide dismutase from the anaerobe Bacteroides distasonis. J Bacteriol. 1977 Mar;129(3):1298–1302. doi: 10.1128/jb.129.3.1298-1302.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haugaard N. Cellular mechanisms of oxygen toxicity. Physiol Rev. 1968 Apr;48(2):311–373. doi: 10.1152/physrev.1968.48.2.311. [DOI] [PubMed] [Google Scholar]
- Hewitt J., Morris J. G. Superoxide dismutase in some obligately anaerobic bacteria. FEBS Lett. 1975 Feb 15;50(3):315–318. doi: 10.1016/0014-5793(75)80518-7. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Loesche W. J. Oxygen sensitivity of various anaerobic bacteria. Appl Microbiol. 1969 Nov;18(5):723–727. doi: 10.1128/am.18.5.723-727.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lumsden J., Hall D. O. Superoxide dismutase in photosynthetic organisms provides an evolutionary hypothesis. Nature. 1975 Oct 23;257(5528):670–672. doi: 10.1038/257670a0. [DOI] [PubMed] [Google Scholar]
- Marklund S., Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974 Sep 16;47(3):469–474. doi: 10.1111/j.1432-1033.1974.tb03714.x. [DOI] [PubMed] [Google Scholar]
- McBEE R. H., LAMANNA C., WEEKS O. B. Definitions of bacterial oxygen relationships. Bacteriol Rev. 1955 Mar;19(1):45–47. doi: 10.1128/br.19.1.45-47.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCord J. M., Keele B. B., Jr, Fridovich I. An enzyme-based theory of obligate anaerobiosis: the physiological function of superoxide dismutase. Proc Natl Acad Sci U S A. 1971 May;68(5):1024–1027. doi: 10.1073/pnas.68.5.1024. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore W. E., Cato E. P., Holdeman L. V. Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections. J Infect Dis. 1969 Jun;119(6):641–649. doi: 10.1093/infdis/119.6.641. [DOI] [PubMed] [Google Scholar]
- Moore W. E., Holdeman L. V. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol. 1974 May;27(5):961–979. doi: 10.1128/am.27.5.961-979.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherman J. M. THE PRODUCTION OF CATALASE BY AN ANAEROBIC ORGANISM. J Bacteriol. 1926 Jun;11(6):417–418. doi: 10.1128/jb.11.6.417-418.1926. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steingrimsson O., Ryan R. W., Tilton R. C. The microdilution antibiotic susceptibility test. Bacteroides fragilis. Am J Clin Pathol. 1976 Jun;65(6):1010–1015. doi: 10.1093/ajcp/65.6.1010. [DOI] [PubMed] [Google Scholar]
- Tally F. P., Goldin B. R., Jacobus N. V., Gorbach S. L. Superoxide dismutase in anaerobic bacteria of clinical significance. Infect Immun. 1977 Apr;16(1):20–25. doi: 10.1128/iai.16.1.20-25.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tally F. P., Stewart P. R., Sutter V. L., Rosenblatt J. E. Oxygen tolerance of fresh clinical anaerobic bacteria. J Clin Microbiol. 1975 Feb;1(2):161–164. doi: 10.1128/jcm.1.2.161-164.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamada T., Carlsson J. Regulation of lactate dehydrogenase and change of fermentation products in streptococci. J Bacteriol. 1975 Oct;124(1):55–61. doi: 10.1128/jb.124.1.55-61.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]