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. 2003 Feb 10;88(3):388–395. doi: 10.1038/sj.bjc.6600736

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Copyright © 2003 Cancer Research UK

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Western blot analysis of SV40 Tag. Cell extracts were immunoprecipitated with an anti-p53 antibody and the resulting immunoprecipitates were immunobloted with anti-Tag antibody as described in Materials and Methods. None of the six HMCLs investigated here and HL60 (negative control) presented a band corresponding to SV40 Tag, whereas the positive control (SV40) showed the expected band at 94 kDa.