Table 2. Enhanced sensitivity to etoposide and doxorubicin in serum-free media.
%G0–G1 | %S | %G2–M | |
---|---|---|---|
Media alone | |||
Parental | 54 | 36 | 10 |
wtCD26 | 55 | 34 | 11 |
Etoposide (0.25 μM) | |||
Parental | 51 | 30 | 19 |
wtCD26 | 34 | 23 | 43 |
Etoposide (0.50 μM) | |||
Parental | 36 | 28 | 36 |
wtCD26 | 18 | 18 | 64 |
Doxorubicin (0.10 μM) | |||
Parental | 43 | 30 | 27 |
WtCD26 | 31 | 20 | 49 |
Doxorubicin (0.25 μM) | |||
Parental | 36 | 23 | 41 |
wtCD26 | 14 | 12 | 74 |
Following pretreatment with AIM V serum-free media at 37°C for 24 h, wtCD26 Jurkat transfectants and parental cells were incubated at 37°C in serum-free media with etoposide or doxorubicin for 24 h. Cells were then harvested and cell cycle analyses were performed with PI staining. Data are representative of three separate experiments.