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. 2003 Apr 15;88(8):1271–1276. doi: 10.1038/sj.bjc.6600891

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Copyright © 2003 Cancer Research UK

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Example of an RSM experiment involving the Msp I restriction enzyme of codon 248 and DNA extracted from nine Barrett's tissues. The right-hand lane contains a positive control for the PCR step and shows the expected PCR band size. The left-hand lane contains a DNA ladder showing the 100, 200 and 300 bp bands. The central lanes contain DNA from Barrett's biopsies subject to RSM analysis. The highlighted lane contains an undigested band of the correct size and was subject to DNA sequencing where it was confirmed that it contained a GC to AT mutation. The remaining lanes (including the internal digestion control situated next to the PCR-positive control) have digested and hence do not contain mutations in this restriction site (codon 248).