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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Virology. 2009 Jun 13;390(2):330–337. doi: 10.1016/j.virol.2009.05.013

Fig. 4. cmvIL-10 inhibits IFN-expression but not maturation of PDCs.

Fig. 4

Freshly isolated PDCs were left untreated or stimulated with CpG in the presence or absence of 5 ng/ml hIL-10 or cmvIL-10 for 18 h. The surface expression of indicated molecules on PDCs was assessed by FACS and is presented with overlaid histograms. Shown are representative data from one of five individual donors that gave similar results (A). IL-10 significantly reduces type I IFN production by CpG-activated PDCs. IFN-α (upper panel, n = 5) or IFN-β (lower panel, n = 3) protein concentrations in supernatants were measured by ELISA. Each dot represents the relative concentrations (%) in the cultures compared to CpG-only stimulation from individual donors. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post tests (***p < 0.001) (B).