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. Author manuscript; available in PMC: 2010 Feb 18.
Published in final edited form as: J Am Chem Soc. 2009 Feb 18;131(6):2359–2366. doi: 10.1021/ja8081044

Figure 2.

Figure 2

Binding affinities determined through DNA photocleavage. The DNA hairpin sequence is 5′-GGCAGGXATGGCTTTTTGCCATCCCTGCC-3′ (X = C or A, underline denotes the mismatch). Samples were irradiated and electrophoresed through a 20% denaturing PAGE gel. A light control (LC, without rhodium) and dark control (DC, without irradiation) were included. A representative autoradiogram of a photocleavage titration with rac-Rh(bpy)2chrysi3+ (A, arrows indicate positions of mismatched bases) and a representative sigmoidal curve fit of pooled data from photocleavage titrations for binding constant determination (B) are shown.