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. Author manuscript; available in PMC: 2010 Feb 18.
Published in final edited form as: J Am Chem Soc. 2009 Feb 18;131(6):2359–2366. doi: 10.1021/ja8081044

Figure 3.

Figure 3

Inhibitory effects of Rh(NH3)4chrysi3+ as a function of incubation time on cellular proliferation. Shown are plots of BrdU incorporation (a measure of DNA synthesis and therefore cellular proliferation) normalized to the BrdU incorporation of untreated cells as a function of rhodium concentration. Standard error bars for five trials are shown. MMR-proficient HCT116N cells (green) and MMR-deficient HCT116O cells (red) were plated and allowed 24 h to adhere before incubation with 0–25 μM Rh(NH3)4chrysi3+ for 12, 24, 48, or 72 h. At the end of the 12, 24, and 48 h incubations, the medium containing Rh was replaced with fresh medium for the remainder of the 72 h, followed by ELISA analysis. BrdU was added to the medium 24 h prior to analysis.