Table 1.
Primer sequences for quantitative PCR. When possible, primer sets were designed over exon boundaries to prevent amplification of genomic DNA. Under Primer sequences, the primers used for quantitation are labeled F=forward and R=reverse. Position refers to numbering within coding sequence (CDS) or expressed sequence tag (EST). Exon boundaries are predicted based on homology with zebrafish (Danio rerio).
| Gene name (Symbol, accession number) |
Primer sequences | Position | Exon boundary |
|---|---|---|---|
|
vitellogenin 1 (vtg1, AF130354) |
F 5′- GCTGCAGAGGCCATTTCTAAGA -3′ R 5′- AGCATTGCCCAGAACTTTCAG -3′ |
1393- 1461 |
none |
|
cytochrome P450, family 19, subfamily A, polypeptide 1b (cyp19a1b, AJ277866) |
F 5′- GGACGTTTCCAATAGACTCTTCCTAA -3′ R 5′- ATAGCGATGGATCTTTATCAGCAA -3′ |
585-657 | 625 |
|
cytochrome P450, family 19, subfamily A, polypeptide 1a (cyp19a1a, AF288755) |
F 5′- GGAGAGCTGAGCGCTGAGA -3′ R 5′- GGAGCCGCGATCAACATCT -3′ |
181-239 | none |
|
estrogen receptor 1 (esr1, AY727528) |
F 5′- AACTCATCTTTGCTCAGGATCTCA -3′ R 5′- AGCCATCCCCTCGACACAT -3′ |
1109- 1173 |
1142 |
|
androgen receptor (ar, AY727529) |
F 5′- GTGCCATGCGCTTCCAA -3′ R 5′- CTGACCTTTGTGGGCAAGGA -3′ |
2568- 2718 |
2647 |
|
protein inhibitor of activated STAT, 2 (pias2, DT238876.1) |
F 5′- CAGACCCTGACAGTGAAATTGC -3′ R 5′- GCTCGACAGGGTACCGTAAGG -3′ |
94-184 | 149 |
|
hydroxysteroid (17β)dehydrogenase 12a (hsd17b12a, DT161033.1) |
F 5′- AATTCTTCCTCCACATCCCTGAT dehydrogenase -3′ R 5′- TCATTTGGCACACTGAGGTGAT -3′ |
260-340 | 291 |
|
zona pellucida glycoprotein 2.2 (zp2.2, DT088130.1) |
F 5′- ACGAGGAGGTCATTCTGTTTTCA -3′ R 5′- TGGGATCCACACCTTTCTTTG -3′ |
636-746 | 682 |
|
hypothetical protein LOC565514 (loc565514, DT346207 (5′ EST)) |
F 5′- GCTGTGTTTGTGGAGCCGTTA -3′ R 5′- GTCTGGGACTCTTGGTCATCATC -3′ |
207-287 | none |
|
hypothetical protein LOC565514 (loc565514, DT306961 (3′ EST)) |
F 5′- ACACAATCTCATTCAGTGAAATCCTT -3′ R 5′- TGAGTGCTTGGGTATGAGAAAACT -3′ |
328-408 | none |
|
hypothetical protein LOC565514 (loc565514, DT306961 (3′ EST)) |
F 5′- GCAGAGCATCTGCATACATCTTAATT -3′ R 5′- TGTGAGCCCCTTGAGAGAGAGTA -3′ |
34-156 | none |
|
clusterin (clu, UniGene Ppr.6588) |
F 5′- ACGAGGATGTTGTTATATCGAAACC -3′ R 5′- AATTGCGGCGGATTTCC -3′ |
957- 1025 |
990 |
|
prostaglandin D2 synthase (ptgds, UniGene Ppr.12268) |
F 5′- TTGGACACCGGCATCCTT -3′ R 5′- TTTAAGAGACCCTCAGGCATCTG -3′ |
540-620 | 589 |
|
zgc:55572 (zgc:55572, UniGene Ppr.6860) |
F 5′- TGCGCAAGGCTGATATGCT -3′ R 5′- GACTGTCTGCCTCCACAAGGA -3′ |
385-465 | 417 |
|
ribosomal protein L8 (rpl8, AY919670) |
F 5′- CATACCACAAGTACAAGGCCAAGA -3′ R 5′- ACCGAAGGGATGCTCAACAG -3′ |
516-598 | 577 |