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Calmodulin enhances Hsp90 inhibition of nNOS ubiquitination. nNOS was preincubated with Hsp70/Hsp40 in the presence or absence of Hsp90, CaM, EGTA, or geldanamycin (GA) as noted at the top of the figure. The samples were then incubated with the ubiquitinating enzyme mixture and Western blotted by probing with anti-nNOS.
