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Calmodulin does not inhibit and Hsp90 does inhibit nNOS ubiquitination by Fraction A of reticulocyte lysate. nNOS was preincubated with 30 μM CaM and/or Hsp90 as indicated and then incubated with Hsp90-free Fraction A of reticulocyte lysate. The samples were Western blotted by probing with anti-nNOS.
