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. Author manuscript; available in PMC: 2010 Sep 8.
Published in final edited form as: Biochemistry. 2009 Sep 8;48(35):8483–8490. doi: 10.1021/bi901058g

FIGURE 6.

FIGURE 6

Hsp90 inhibits the guanabenz-triggered ubiquitination of nNOS. A, inhibition of ubiquitination. nNOS was preincubated with Hsp70/40 under conditions required for enzyme activity and in the presence of guanabenz and/or Hsp90 as noted at the top of figure. The samples were then incubated with the ubiquitinating enzyme mixture and Western blotted for nNOS. B, guanabenz inhibition of nNOS activity. nNOS was preincubated as in A and nNOS activity was assayed by measuring the NO-mediated conversion of oxyhemoglobin to methemoglobin as described under Methods. Bars represent mean ± S.E. for three experiments.