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Hsp90 inhibits NAA-triggered ubiquitination of nNOS. nNOS was preincubated with Hsp70/40 under conditions required for enzyme activity (see Methods) in the presence of 100 μM NAA and/or Hsp90 as noted at the top of the figure. The samples were then incubated with the ubiquitinating enzyme mixture and Western blotted for nNOS.
