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. Author manuscript; available in PMC: 2009 Sep 21.
Published in final edited form as: DNA Repair (Amst). 2007 Jun 7;6(10):1453–1462. doi: 10.1016/j.dnarep.2007.04.009

Fig. 4.

Fig. 4

Subnuclear localization of hS3 in cells treated with hydrogen peroxide. Cytosol (C) and nuclear fractions were isolated from HEK 293 cells exposed to vehicle alone or 0.125 mM hydrogen peroxide for 24 h. Purified nuclei were further subjected to subfractionation as described under Experimental Procedures. Cytosol as well as various subnuclear fractions (Nuclease supernatant (N•), Triton X-100 extraction (T), NaCl extraction (N*), Subnuclear matrix (M) were separated by SDS-PAGE and immunoblotted against hS3 (top panel). The membrane was further stripped and re-probed with the anti-PARP antibody.