Figure 2.

Functional characterization of shPARG and shPARP1 cells. Oxidative stress-induced PAR accumulation in A549, control, shPARP1-, and shPARG cells was detected by immunocytochemistry (A) or immunoblotting (B). Following treatment with 400 μM hydrogen peroxide, PAR polymers were detected immediately (0 time) or after increasing recovery times. β-Actin was used as the loading control (B). Lack of PAR polymer synthesis was observed in shPARP1 cells, while delayed PAR polymer degradation was detected in shPARG cells.