Figure 6.

The envelope-associated protein Emp is a component of the extracellular matrix in staphylococcal biofilms. A) Coomassie-stained SDS-PAGE identified envelope-associated proteins Eap and Emp and characterized a bursa aurealis mutant (emp) with (pEmp) and without complementing plasmid. B–D) Expression of Emp in the extracellular matrix of in vitro-formed biofilms was detected by microscopy, using BODIPY-vancomycin-stained staphylococci (green) and AlexaFluor-647-labeled secondary antibody to recognize the deposition of rabbit anti-Emp (red).