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. 2004 Aug 3;91(6):1205–1212. doi: 10.1038/sj.bjc.6602079

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Copyright © 2004 Cancer Research UK

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Topoisomerase I analysis by Western blot at passages 1, 5, 11 and 13. Protein from tissue homogenates, 50 μg, were separated in 7.5% SDS–polyacrylamide gels, transferred to a nitrocellulose membrane and immunoblotted with human polyclonal topoisomerase I antibody (Topogen Inc.).