Skip to main content
. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Protein Expr Purif. 2008 Dec 30;65(2):230–237. doi: 10.1016/j.pep.2008.12.013

Fig. 1.

Fig. 1

Expression of Rv3297 (MtuNei2) in the pET30a vector in the presence of pRARE2 tRNA plasmid. Uninduced (lanes 1, 3 and 5) and induced (lanes 2, 4 and 6) whole cell lysates of transformed BL21-Gold (DE3) cells were analyzed on a 12% SDS–PAGE gel. Lane M, Novagen Perfect Protein™ Marker (15–150 kDa). Lane P, purified MtuNei2-His (29.6 kDa).