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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Protein Expr Purif. 2008 Dec 30;65(2):230–237. doi: 10.1016/j.pep.2008.12.013

Fig. 2.

Fig. 2

Schematic of the bicistronic vectors used in this study. (a) Parental pET30a-ORF6 vector, (b) Rv3297 cloned into the pET30a-ORF6. RBS for the upstream leader sequence and the downstream target gene are boxed. The cloning sites for the Rv3297 (MtuNei2) gene are underlined in the parental vector. Nucleotides ‘AT’ of the EcoRV recognition sequence overlap with the stop codon for the upstream ORF6 and the initiation codon for the downstream target gene.