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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Protein Expr Purif. 2008 Dec 30;65(2):230–237. doi: 10.1016/j.pep.2008.12.013

Fig. 3.

Fig. 3

Induction of Rv3297 (MtuNei2) expression in pET30a and the pET30a-ORF6 bicistronic vector in the presence of pRARE tRNA plasmid or pACYC184 missing the tRNA genes. Uninduced (lanes 1, 3, 5, 7 and 9) and induced (lanes 2, 4, 6, 8 and 10) whole cell lysates of transformed BL21-Gold (DE3) cells were analyzed on a 12% SDS–PAGE. Lane M, Novagen Perfect Protein™ Marker (15–150 kDa). Lane P, purified MtuNei2-His (29.6 kDa).