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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Protein Expr Purif. 2008 Dec 30;65(2):230–237. doi: 10.1016/j.pep.2008.12.013

Fig. 6.

Fig. 6

Induction of sRv2464 (MtuNei1) and cRv2924 (MtuFpg1) expression in the pET30a-ORF6 bicistronic vector. Uninduced (lanes 1 and 3) and induced (lanes 2 and 4) whole cell lysates of transformed BL21-Gold (DE3) cells were analyzed on a 12% SDS–PAGE. Lane M, Novagen Perfect Protein™ Marker (15–150 kDa). Lane P1, purified MtuNei1-His (30.8 kDa). Lane P2, purified MtuFpg1-His (33.0 kDa).