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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Immunother. 2009 Sep;32(7):755–764. doi: 10.1097/CJI.0b013e3181ab1807

Table 1.

Sequences of primers and probes used in real-time quantitative-PCR analysis

Gene: Sequences:

CD25 Forward: AATGCAGCCAGTGGACCAA
Reverse: TGATAAATTCTCTCTGTGGCTTCATTT
Probe: CCAGGTCACTGCAGGGAACCTCCAC

IFN-γ Forward: GAAAAGCTGACTAATTATTCGGTAACTG
Reverse: GTTCAGCCATCACTTGGATGAG
Probe: TTGAATGTCCAACGCAAAGCAATACATGA

FoxP3 Forward: AACAGCACATTCCCAGAGTTCCT
Reverse: CATTGAGTGTCCGCTGCTTCT
Probe: CCTTTCACCTACGCCACGCTCATCC

IL-10 Forward: GAGGCTACGGCGCTGTCAT
Reverse: AGATGCCTTTCTCTTGGAGCTTATT
Probe: CAAGAGCAAGGCCGTGGAGCAGG

CTLA-4 Forward: CGCCATACTACCTGGGCATAG
Reverse: AGGATCCAGAGGAGGAAGTCAGA
Probe: AGATTTATGTAATTGATCCAGAACCGTG