Skip to main content
. Author manuscript; available in PMC: 2009 Sep 21.
Published in final edited form as: J Neurochem. 2007 Nov 5;105(1):68–77. doi: 10.1111/j.1471-4159.2007.05110.x

Fig. 6.

Fig. 6

Inhibition of N-linked glycosylation abolishes the increased heteromerization of HCN1 channels by seizure-like network activity. (a) Quantitative analysis of the extent of heteromerization of HCN1 channels in organotypic hippocampal slice cultures was carried out using co-immunoprecipitation (IP), calibrated to the total amount of the HCN1 channels in the tissue. Actin was used to compare protein content among groups. As shown in the representative gels, in the absence of tunicamycin seizure-like activity (SA) increased the optical density of the HCN1 immunoreactive band that precipitated with antiserum to HCN2. However, after cultures were maintained in Tunicamycin (see text and Fig. 5), SA no longer enhanced the co-association of HCN1/HCN2 channels. (b) Quantitative analysis of the percentage of heteromerization (n = 5 per group).