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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Anal Chem. 2009 Jul 1;81(13):5426–5432. doi: 10.1021/ac9005827

Figure 1.

Figure 1

A schematic system layout for the microfabricated flow cytometer for multiplexed detection of bacteria and toxins. A syringe pump was used to provide sheath flow, while a CAVRO pump was used to inject samples into the microfabricated channel. The cables inserted into the PDMS chip guided 635 and 532 nm laser light into the interrogation region and guided excess light out of the beam stops.11 More fiber optics directed emission light to four separate PMTs to collect microsphere ID fluorescences (670 ± 10 nm and ≥700 nm), light scatter (635 ± 5 nm), and phycoerythrin fluorescence (565 ± 10 nm). Sizes are not to scale.