Table 1.
LODs for the Three Bacteria Species and Three Toxins and Their Associated Coefficients of Variation (CV) Were Determined Using the Conventional Benchtop Cytometer (Luminex) and the Microflow Cytometer before and after Signal Amplificationa
| bacteria (cfu/mL) |
toxins (ng/mL) |
|||||
|---|---|---|---|---|---|---|
| cytometer | E. coli | Listeria | Salmonella | cholera | SEB | ricin |
| Luminex (% CV) | 103 (1.0) | 106 (0.7) | 105 (2.7) | 1.6 (1.0) | 0.064 (2.5) | 8.0 (5.6) |
| Luminex (amplified) (% CV) | 103 (3.2) | 106 (0.5) | 105 (1.4) | 1.6 (2.6) | 0.064 (6.6) | 8.0 (2.3) |
| Microflow (% CV) | 104 (11) | 105 (14) | 105 (28) | 0.32 (22) | 1.6 (7.7) | 1.6 (6.7) |
| Microflow (amplified) (% CV) | 103 (21) | 105 (28) | 104 (34) | 1.6 (15) | 0.064 (23) | 1.6 (19) |
a
Limits of detection were designated as the tested concentration of the antigen that produced a mean signal greater than the average of the mean signals from the five microsphere sets that did not specifically bind the tested antigen (negative controls) plus 3 times the standard error. The coefficients of variation (CVs) were determined for the analyses at the LODs.