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. 2009 Jul 17;8(9):1330–1340. doi: 10.1128/EC.00092-09

FIG. 5.

FIG. 5.

Immunoelectron micrographs depicting the association of GFP-PfSec22 with transition vesicles and tubovesicular elements. Ultrathin cryosections of the GFP-PfSec22-expressing cells were probed with monoclonal anti-GFP antibodies, followed by immunogold detection using gold (12 nm)-labeled anti-mouse secondary antibodies. (A) Association of GFP-PfSec22 with ER-derived transition vesicles (arrows). (B) Association of GFP-PfSec22 with membrane-limited vesicles (arrows) in the host cell compartment. (C) Association of GFP-PfSec22 with the TVN-like extension (dashed arrow) and membrane-bound vesicles (solid arrows) in the infected erythrocyte cytosol. N, nucleus; MC, Maurer's cleft; RBC, red blood cell cytosol. Scale bars, 500 nm (A) and 100 nm (B and C).