FIG. 11.

Cells can translocate anteriorly in a persistent fashion when adhering to the substrate only by their uropod. Cells were incubated in Tricine buffer containing 80 mM CaCl₂, which has been shown to block selectively adhesion to a substratum by the main cell body and anterior pseudopod, but not adhesion of the uropod (46). (A) Track of the posterior tip of a representative cell imaged through differential interference contrast optics at the substratum. (B) Track of the posterior tip of 3D reconstructions observed from on top (90°). (C) Track of the posterior tip of 3D reconstructions observed from the side (0°). The original position (asterisk) and translocation track (dotted line) of the posterior tip are indicated. s, seconds.