FIG. 1.

Tbμ1 silencing in trypanosomes. Transgenic PCF and BSF cell lines containing the Tbμ1 RNAi construct were cultured without (tet−) or with (tet+; 1 μg/ml) tetracycline to initiate specific dsRNA synthesis. (A) Growth. Cell density was monitored by microscopic counting, and cultures were adjusted to starting densities every 2 days (PCF) or 1 day (BSF). Data from triplicate cultures are presented as means ± SEM. The arrowheads indicate time points (PCF, day 3; BSF, day 1) for all subsequent RNAi phenotypic analyses. (B) Northern analyses. As indicated, Tbμ1 mRNA levels were determined in control PCF (P) and BSF (B) cells and in PCF and BSF cell lines containing the Tbμ1 RNAi construct after culture without (−) or with (+) tetracycline to induce dsRNA synthesis. The blots were first probed for Tbμ1 (top blots) and then stripped and reprobed for tubulin (bottom blots) as a loading control. All lanes contained 5 μg total RNA. Phosphorimages are presented. Size is indicated in kilobases.