FIG. 5.

Silencing of clathrin heavy chain in PCF trypanosomes. A PCF cell line containing the clathrin heavy-chain RNAi construct was cultured without (tet−) and with (tet+) tetracycline to induce synthesis of specific dsRNA. (A) Growth was monitored by microscopy for triplicate experiments (means ± SEM), and the arrowhead indicates the time point for all subsequent RNAi phenotypic analyses. Northern analyses (inset) were performed as for Fig. 1B. (B) Transport and processing of trypanopain were monitored as described in the legend to Fig. 3. Cell-associated (circles) and secreted (squares) trypanopain was quantified for triplicate experiments and is presented as a percentage of initial trypanopain (means ± SEM).