FIG. 6.

Effect of Tbμ1 silencing on protein trafficking in BSF trypanosomes. BSF cell lines containing the Tbμ1 dsRNA construct were assayed for transport of the transgenic reporter BiPN (A) and for endogenous VSG221 (B), p67 (C), and trypanopain (D) (means ± SEM). Control (tet−) and silenced (tet+; 24 h) cells were analyzed by standard pulse-chase analysis for the various reporters. Note that the maximum secreted BiPN seen in BSF cells was always ∼50% (48) and that the endogenous VSG transport assay used in BSF trypanosomes was different from that used in transgenic PCF cells (see the text).