FIG. 1.

Procyclin localizes predominantly to the lysosomes in TbGPI12 null cells. Immunofluorescence microscopy of wild-type (WT) and TbGPI12 null (Null) procyclic trypanosomes stained with anti-EP procyclin (panels A and E; the TbGPI12 null mutant and its wild-type parent cell line express almost exclusively EP procyclins [25]) and anti-trypanopain (panels B and F) antibodies, as indicated. The merged images (panels C and G) and merged images together with the corresponding phase-contrast images (panels D and H) are also shown. Trypanopain is a lysosomal marker (51).