FIG. 3.

Radiolabeling of a high-apparent-MW glycoconjugate fraction with ³H-monosaccharides. Wild-type (WT) and TbGPI12 null (Null) procyclic trypanosomes were biosynthetically labeled with [³H]glucosamine ([3H]GlcN; panel A, lanes 1 and 2), [³H]glucose ([3H]Glc; panel B, lanes 3 and 4) and [³H]mannose ([3H]Man; panel C, lanes 5 and 6), as indicated. Cell lysates were analyzed by SDS-PAGE and fluorography. The lysates of the [³H]mannose-labeled TbGPI12 null cells were also subjected to a pulldown on ricin-agarose (panel D, lane 7). The positions of the MW markers are shown on the left.