FIG. 5.

Purification of the high-MW glycoconjugate fraction. After ricin affinity chromatography, the majority of the high-MW glycoconjugate fraction was eluted in fractions 18 to 20 from a Superose 6B gel filtration column. Aliquots of fractions 17 to 20, corresponding to molecules with a predicted molecular mass of 6 MDa to 600 kDa, were analyzed by SDS-PAGE and stained for protein with Coomassie blue or for carbohydrate by periodate-Schiff (Per.-Schiff). A sample of fraction 18 was also subjected to ricin blotting. The positions of the MW markers are shown on the left.