TABLE 3.
Monosaccharideb | Amt of protein (nmol/mg)c | % by wt |
---|---|---|
Mannose | 175 ± 25 | 2.8 |
Galactose | 162 ± 19 | 2.6 |
Fucose | 83 ± 16 | 1.2 |
N-Acetylglucosamine | Present but not quantitatedd | N/Ae |
Pooled material (fractions 18 to 20) from the gel filtration step of the purification was analyzed for protein content by colorimetric assay and monosaccharide content by GC-MS.
Glucose and a trace of xylose were also detected, but these are common contaminants of biological preparations and have been omitted from the table.
Means and standard deviations of the results from triplicate analyses.
The N-acetylglucosamine derivatives were positively identified by retention time and mass spectrum, but quantification of these derivatives is unreliable.
N/A, not applicable.