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Two-color FISH assay for the identification of C. parvum and C. hominis oocysts, carried out using Cpar677-Cy3 and Chom253-FITC. (A) Positive hybridization of C. hominis oocysts was revealed by bright-green fluorescence. (B) Positive hybridization of C. parvum oocysts was revealed by bright-red fluorescence. (C) A lack of fluorescence confirms FISH-negative oocysts.
